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Paleontologists Debate Whether New Research Found Signs of DNA in Dinosaur Fossil

The fossils are several times older than the theoretical maximum shelf life of DNA molecules

Correspondent

March 6, 2020

Dino babies — Reconstruction of the nesting ground of Hypacrosaurus stebingeri from the Two Medicine formation of Montana. In the center can be seen a deceased Hypacrosaurus nestling with the back of its skull embedded in shallow waters. A mourning adult is portrayed on the right. ©Science China Press, Art by Michael Rothman

About 75 million years ago, a nest of plant-eating dinosaurs called Hypacrosaurus stebingeri died in what’s now Montana. Their fossils were found in the 1980s, and now an international team of scientists has presented evidence that the old bones contain traces of genetic material.

The paper published in National Science Review takes a close look at skull shards that would have been made of soft cartilage, instead of bone, in the young dinosaurs. The discovery is small in size, but hugely controversial among paleontologists: what appears to be microscopic cells, the building blocks of complex life, with dark clumps in the middle. A zoomed-in look at one possible cell’s dark spot reveals what the researchers suspect is genetic material.

Study author Alida Bailleul, a paleontologist at the Chinese Academy of Sciences in Beijing, first found the microscopic orbs in 2010 while a student at the Museum of the Rockies, and quickly recognized their resemblance to cells. “I freaked out a little bit—moving away from the microscope, thinking, moving back to the microscope,” she tells Michael Greshko at National Geographic. “I was like, Oh my god, that can’t be, there’s nothing else they can be!”

Fossil microscopy — Photographs of the suspected cells in the nestling's skull fragment. On the left, it appears that two cells are dividing, and the dark region resembles a cell nucleus, where DNA is stored. In the middle, what appears like strands of DNA. On the right, dye fluoresces red indicating chemicals like DNA. ©Science China Press, Photo by Alida Bailleul and Wenxia Zheng

After getting a second opinion from Mary Schweitzer, a paleontologist at North Carolina State University and first author on the paper, the team moved forward analyzing their find. It was surprising because tiny structures like cells and DNA—the molecular twisted-ladder that carries a cell's blueprint—are notoriously fragile. High heat or acidity can destroy them, and so they require a lot of upkeep while an animal is alive, and when it dies, the delicate bits are at the whims of the environment.

If the researchers have found fossilized cells and DNA, they would be several tens of times older than both any found before, and the theoretical preservation limits of the materials, paleontologist Evan Saitta, who works at the Integrative Research Center at the Field Museum of Natural History in Chicago, explains to George Dvorsky at Gizmodo.

Cartilage lacks pores, so Bailleul and her colleages suggest that it may have defended the microscopic structures from the outside environment, the researchers say.

“Fossilized, calcified cartilage may be an ideal place to search for exceptionally preserved biomolecules in other fossils, as this tissue may be less prone to contamination and internal decay than bone,” Royal Ontario Museum paleontologist David Evans, who wasn’t involved in the new study, tells National Geographic. “In calcified cartilage, the cells become trapped and isolated in their matrix and are more likely to be preserved in a sealed micro-environment.”

To check their find, the researchers applied a dye to the fossils that sticks to DNA and fluoresces red. Then, they dyed living emu cells and compared the two. Although it was much fainter than the dye in the emu's cells, the fossil's dye stuck to something.

“I’m not even willing to call it DNA because I’m cautious, and I don’t want to overstate the results,” Schweitzer tells National Geographic. “There is something in these cells that is chemically consistent with and responds like DNA.”

The researchers would have to extract the DNA-like stuff from the fossil and try to read its chemical code in order to definitively confirm whether or not it’s DNA, but based on their results, the pieces are too short to read. It also may have become extra stable by binding to itself and other molecules nearby, a reaction called cross-linking, they tell National Geographic.

Skeptics like University of Bristol paleontologist Michael Benton aren’t convinced that what the researchers found originated in the skull fragment at all. Writing on the Conversation, Benton suggests that the potential DNA could have come from modern contamination. That’s what happened with many claims of ancient DNA in the 1990s, though those studies used a different testing technique than Schweitzer’s team.

He also points out that Schweitzer has co-authored several studies on soft tissue fossils that have been controversial in their field. Saitta’s doubt also comes from the fact that dyes like those used by the researchers sometimes give false positives, indicating their target is present when it’s actually not, he tells Gizmodo.

Schweitzer disagrees, and tells Gizmodo that skeptics “can say what they want,” adding that “As far as I know, [the dyes] DAPI and PI do not bind to any other molecule except DNA.” Unless the skeptics can come up with a better explanation for the data, she’s confident in her team’s conclusions.

“This research is still very much in its infancy,” Evans tells National Geographic. “But the possibilities are absolutely thrilling if we suspend our disbelief, dig into the data, and continue to test and refine our ideas about molecular preservation in fossils.”